Development of a novel real-time PCR-based strategy for simple and rapid molecular pathotyping of Newcastle disease virus
Identifieur interne : 002384 ( Main/Exploration ); précédent : 002383; suivant : 002385Development of a novel real-time PCR-based strategy for simple and rapid molecular pathotyping of Newcastle disease virus
Auteurs : Alia Yacoub [Suède] ; Mikael Leijon [Suède] ; Michael J. Mcmenamy [Royaume-Uni] ; Karin Ullman [Suède] ; John Mckillen [Royaume-Uni] ; Gordon Allan [Royaume-Uni] ; Sándor Belák [Suède]Source :
- Archives of Virology [ 0304-8608 ] ; 2012-05-01.
Abstract
Abstract: A novel real-time PCR strategy was applied to simultaneously detect and to discriminate low-pathogenic lentogenic and virulent meso/velogenic Newcastle disease virus (NDV). The pathotyping is achieved by a three-step semi-nested PCR. A pre-amplification of the cleavage site (CS) region of the F gene is followed by a two-level duplex real-time PCR directly targeting the CS, combining detection and pathotyping in a single tube. A wide range of NDV isolates spanning all genotypes were successfully detected and pathotyped. Clinical samples from outbreaks in Sweden in 2010 that were positive by the novel PCR method were also successfully pathotyped. The method is time-saving, reduces labour and costs and provides opportunities for rapid diagnosis at remote locations and in the field. Since the same strategy was also recently applied to avian influenza virus pathotyping, it shows promise of finding broad utility in diagnostics of infectious diseases caused by different RNA viruses in various hosts.
Url:
DOI: 10.1007/s00705-012-1231-0
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001892
- to stream Istex, to step Curation: 001892
- to stream Istex, to step Checkpoint: 000401
- to stream Main, to step Merge: 002409
- to stream Main, to step Curation: 002384
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of a novel real-time PCR-based strategy for simple and rapid molecular pathotyping of Newcastle disease virus</title><author><name sortKey="Yacoub, Alia" sort="Yacoub, Alia" uniqKey="Yacoub A" first="Alia" last="Yacoub">Alia Yacoub</name></author><author><name sortKey="Leijon, Mikael" sort="Leijon, Mikael" uniqKey="Leijon M" first="Mikael" last="Leijon">Mikael Leijon</name></author><author><name sortKey="Mcmenamy, Michael J" sort="Mcmenamy, Michael J" uniqKey="Mcmenamy M" first="Michael J." last="Mcmenamy">Michael J. Mcmenamy</name></author><author><name sortKey="Ullman, Karin" sort="Ullman, Karin" uniqKey="Ullman K" first="Karin" last="Ullman">Karin Ullman</name></author><author><name sortKey="Mckillen, John" sort="Mckillen, John" uniqKey="Mckillen J" first="John" last="Mckillen">John Mckillen</name></author><author><name sortKey="Allan, Gordon" sort="Allan, Gordon" uniqKey="Allan G" first="Gordon" last="Allan">Gordon Allan</name></author><author><name sortKey="Belak, Sandor" sort="Belak, Sandor" uniqKey="Belak S" first="Sándor" last="Belák">Sándor Belák</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:5C9C85566EA2B13D428BADCE6BBB6454F7ED983F</idno><date when="2012" year="2012">2012</date><idno type="doi">10.1007/s00705-012-1231-0</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-CZNN0WP7-4/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001892</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001892</idno><idno type="wicri:Area/Istex/Curation">001892</idno><idno type="wicri:Area/Istex/Checkpoint">000401</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000401</idno><idno type="wicri:doubleKey">0304-8608:2012:Yacoub A:development:of:a</idno><idno type="wicri:Area/Main/Merge">002409</idno><idno type="wicri:Area/Main/Curation">002384</idno><idno type="wicri:Area/Main/Exploration">002384</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Development of a novel real-time PCR-based strategy for simple and rapid molecular pathotyping of Newcastle disease virus</title><author><name sortKey="Yacoub, Alia" sort="Yacoub, Alia" uniqKey="Yacoub A" first="Alia" last="Yacoub">Alia Yacoub</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Virology, Immunobiology and Parasitology, National Veterinary Institute (SVA), Ulls väg 2B, SE-751 89, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>The Joint R&D Division in Virology of SVA and SLU, The OIE Collaborating Centre for the Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine, Ulls väg 2B, SE-751 89, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Suède</country></affiliation></author><author><name sortKey="Leijon, Mikael" sort="Leijon, Mikael" uniqKey="Leijon M" first="Mikael" last="Leijon">Mikael Leijon</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Virology, Immunobiology and Parasitology, National Veterinary Institute (SVA), Ulls väg 2B, SE-751 89, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>The Joint R&D Division in Virology of SVA and SLU, The OIE Collaborating Centre for the Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine, Ulls väg 2B, SE-751 89, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation></author><author><name sortKey="Mcmenamy, Michael J" sort="Mcmenamy, Michael J" uniqKey="Mcmenamy M" first="Michael J." last="Mcmenamy">Michael J. Mcmenamy</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Biological Sciences, Queen’s University Belfast, BT9 7BL, Belfast, Northern Ireland</wicri:regionArea><wicri:noRegion>Northern Ireland</wicri:noRegion></affiliation></author><author><name sortKey="Ullman, Karin" sort="Ullman, Karin" uniqKey="Ullman K" first="Karin" last="Ullman">Karin Ullman</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Virology, Immunobiology and Parasitology, National Veterinary Institute (SVA), Ulls väg 2B, SE-751 89, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation></author><author><name sortKey="Mckillen, John" sort="Mckillen, John" uniqKey="Mckillen J" first="John" last="Mckillen">John Mckillen</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Veterinary Sciences Division, Agri-Food and Biosciences Institute, BT4 3SD, Belfast, Northern Ireland</wicri:regionArea><wicri:noRegion>Northern Ireland</wicri:noRegion></affiliation></author><author><name sortKey="Allan, Gordon" sort="Allan, Gordon" uniqKey="Allan G" first="Gordon" last="Allan">Gordon Allan</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>School of Biological Sciences, Queen’s University Belfast, BT9 7BL, Belfast, Northern Ireland</wicri:regionArea><wicri:noRegion>Northern Ireland</wicri:noRegion></affiliation></author><author><name sortKey="Belak, Sandor" sort="Belak, Sandor" uniqKey="Belak S" first="Sándor" last="Belák">Sándor Belák</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Virology, Immunobiology and Parasitology, National Veterinary Institute (SVA), Ulls väg 2B, SE-751 89, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences (SLU), Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>The Joint R&D Division in Virology of SVA and SLU, The OIE Collaborating Centre for the Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine, Ulls väg 2B, SE-751 89, Uppsala</wicri:regionArea><wicri:noRegion>Uppsala</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="sub">Official Journal of the Virology Division of the International Union of Microbiological Societies</title><title level="j" type="abbrev">Arch Virol</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer Vienna</publisher><pubPlace>Vienna</pubPlace><date type="published" when="2012-05-01">2012-05-01</date><biblScope unit="volume">157</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="833">833</biblScope><biblScope unit="page" to="844">844</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A novel real-time PCR strategy was applied to simultaneously detect and to discriminate low-pathogenic lentogenic and virulent meso/velogenic Newcastle disease virus (NDV). The pathotyping is achieved by a three-step semi-nested PCR. A pre-amplification of the cleavage site (CS) region of the F gene is followed by a two-level duplex real-time PCR directly targeting the CS, combining detection and pathotyping in a single tube. A wide range of NDV isolates spanning all genotypes were successfully detected and pathotyped. Clinical samples from outbreaks in Sweden in 2010 that were positive by the novel PCR method were also successfully pathotyped. The method is time-saving, reduces labour and costs and provides opportunities for rapid diagnosis at remote locations and in the field. Since the same strategy was also recently applied to avian influenza virus pathotyping, it shows promise of finding broad utility in diagnostics of infectious diseases caused by different RNA viruses in various hosts.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li><li>Suède</li></country></list><tree><country name="Suède"><noRegion><name sortKey="Yacoub, Alia" sort="Yacoub, Alia" uniqKey="Yacoub A" first="Alia" last="Yacoub">Alia Yacoub</name></noRegion><name sortKey="Belak, Sandor" sort="Belak, Sandor" uniqKey="Belak S" first="Sándor" last="Belák">Sándor Belák</name><name sortKey="Belak, Sandor" sort="Belak, Sandor" uniqKey="Belak S" first="Sándor" last="Belák">Sándor Belák</name><name sortKey="Belak, Sandor" sort="Belak, Sandor" uniqKey="Belak S" first="Sándor" last="Belák">Sándor Belák</name><name sortKey="Leijon, Mikael" sort="Leijon, Mikael" uniqKey="Leijon M" first="Mikael" last="Leijon">Mikael Leijon</name><name sortKey="Leijon, Mikael" sort="Leijon, Mikael" uniqKey="Leijon M" first="Mikael" last="Leijon">Mikael Leijon</name><name sortKey="Ullman, Karin" sort="Ullman, Karin" uniqKey="Ullman K" first="Karin" last="Ullman">Karin Ullman</name><name sortKey="Yacoub, Alia" sort="Yacoub, Alia" uniqKey="Yacoub A" first="Alia" last="Yacoub">Alia Yacoub</name><name sortKey="Yacoub, Alia" sort="Yacoub, Alia" uniqKey="Yacoub A" first="Alia" last="Yacoub">Alia Yacoub</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Mcmenamy, Michael J" sort="Mcmenamy, Michael J" uniqKey="Mcmenamy M" first="Michael J." last="Mcmenamy">Michael J. Mcmenamy</name></noRegion><name sortKey="Allan, Gordon" sort="Allan, Gordon" uniqKey="Allan G" first="Gordon" last="Allan">Gordon Allan</name><name sortKey="Mckillen, John" sort="Mckillen, John" uniqKey="Mckillen J" first="John" last="Mckillen">John Mckillen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002384 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002384 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:5C9C85566EA2B13D428BADCE6BBB6454F7ED983F
|texte= Development of a novel real-time PCR-based strategy for simple and rapid molecular pathotyping of Newcastle disease virus
}}
| This area was generated with Dilib version V0.6.33. |  |